Composite

Part:BBa_K5040008:Design

Designed by: Chun Ying Yu   Group: iGEM24_CCU-Taiwan   (2024-09-24)


T7promoter-Zif268-rLink-m-hTPH1-PBSII-rlinker-hPCBD1-ZFa-hQDPR-T7terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1362
    Illegal XbaI site found at 39
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1362
    Illegal BglII site found at 403
    Illegal BamHI site found at 1338
    Illegal XhoI site found at 3086
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1362
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1362
    Illegal XbaI site found at 39
    Illegal NgoMIV site found at 2577
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


Design Notes

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Source

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References